| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232123 |
| Chromosome: | chromosome 3 |
| Location: | 1862996 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154750 | (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCTAAAAACGCTGCACACACCTGCATT |
| Internal bar code: | CTCGGTTATTATCGCGGTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1161 |
| LEAP-Seq percent confirming: | 99.726 |
| LEAP-Seq n confirming: | 4003 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCCAGGTGAAAAATCA |
| Suggested primer 2: | AGCCGAAGTCGAAATGCTAA |