| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232157 |
| Chromosome: | chromosome 7 |
| Location: | 1174447 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320750 | CYG12 | Adenylate/guanylate cyclase, nitric oxide sensing; (1 of 5) K12319 - guanylate cyclase soluble subunit beta (GUCY1B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAACGCAGCCGAACCGACGTACCCTGTC |
| Internal bar code: | TACGCGAGTGTTCCATATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 99.2565 |
| LEAP-Seq n confirming: | 1602 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGAGGTGTTGTGAGTGC |
| Suggested primer 2: | AGGTTTTGGGGTAATCAGGG |