Insertion junction: LMJ.RY0402.232160_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.232160
Chromosome:chromosome 7
Location:1100671
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g320000 GT90-38,GT90F38 GT90 family protein 38; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCAACAGTACACATTTCTTGGTCCGGT
Internal bar code:GCGGGGCTTTCAGTCCAACCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:517
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGCTCAACATTTGCGTTC
Suggested primer 2:GTACTTGACTGGCTTTCGGC