| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232162 |
| Chromosome: | chromosome 3 |
| Location: | 3987270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171900 | FAP56 | Flagellar Associated Protein 56 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCGCCAAACCCTGGCGCTTCCCTACC |
| Internal bar code: | CTGTGTGGGTGAAAATAGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 99.1739 |
| LEAP-Seq n confirming: | 2641 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGACACGGTGCCTAGAAT |
| Suggested primer 2: | ACTTGTAGAACGCAATGGGG |