| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232175 |
| Chromosome: | chromosome_6 |
| Location: | 7198923 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g297850 | DHC12 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAATTGAATCCCGAGCGCCCATTCCTGA |
| Internal bar code: | CGACTGTATGTTCGTCTCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 99.6923 |
| LEAP-Seq n confirming: | 2916 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTGCGTATGGATGTTG |
| Suggested primer 2: | GCTCAAGAGAGACACGGAGC |