| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232179 |
| Chromosome: | chromosome 16 |
| Location: | 2264238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCAGGCGGCAGCGGCCGCTTCCCAGCAG |
| Internal bar code: | CGCCGCTGTAAGGGTCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 60 |
| LEAP-Seq percent confirming: | 80.7018 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGAGCCTGTGGTATACGG |
| Suggested primer 2: | CCTCTATTGCTCGCCTGGTA |