| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232184 |
| Chromosome: | chromosome 2 |
| Location: | 4882859 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103350 | (1 of 1) 3.5.1.99 - Fatty acid amide hydrolase / Oleamide hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGATGGGGGCCGCAGCGGTAGGGGGCTC |
| Internal bar code: | CTGGTCCCGCGGCACGTTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.2462 |
| LEAP-Seq n confirming: | 1580 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGACATGGAGACTGGA |
| Suggested primer 2: | CATAAGACGCAACGAAGCAA |