| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232191 |
| Chromosome: | chromosome_12 |
| Location: | 3214438 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g498800 | antisense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTCTGAGCTCTTCTCAAGAGACATGACAA |
| Internal bar code: | AAGATGTCAAGGAGGCAGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 98.2172 |
| LEAP-Seq n confirming: | 606 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGAGATGGACACATGC |
| Suggested primer 2: | CAAACTTCGACGGACATCCT |