| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232201 |
| Chromosome: | chromosome 6 |
| Location: | 6364886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292100 | (1 of 1) PTHR30222//PTHR30222:SF3 - SPERMIDINE/PUTRESCINE-BINDING PERIPLASMIC PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCATTCCGCCCACGCCCTTTCCCCTTA |
| Internal bar code: | GGGAAGCATGGGGGGGGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 98.3406 |
| LEAP-Seq n confirming: | 1126 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTGGAGTGGGAGTAAC |
| Suggested primer 2: | TTGCCGACCAGTCACATAAA |