| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232204 |
| Chromosome: | chromosome 12 |
| Location: | 4076207 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517700 | NYC1,SDR21 | (1 of 2) K13606 - chlorophyll(ide) b reductase (NOL, NYC1); Chlorophyll b reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGCGGCATGTGAAGCGGCCAGCCGCA |
| Internal bar code: | ACCGAAGCCGGGGCCCTTACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTTGTACTCACCTGCG |
| Suggested primer 2: | AATACCCCGCTTGTCAACAG |