| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232204 |
| Chromosome: | chromosome 16 |
| Location: | 4777506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686552 | (1 of 1) 3.6.1.12 - dCTP diphosphatase / Deoxycytidine-triphosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGACGGTTTAGCTCCTACTCAAGTTGG |
| Internal bar code: | TTATATTGACTAGTTGCTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 98.6839 |
| LEAP-Seq n confirming: | 3674 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCCTCACCAGGTACAG |
| Suggested primer 2: | GCGAAGTGTGCTGATTTTGA |