| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232217 |
| Chromosome: | chromosome 11 |
| Location: | 3508227 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481350 | (1 of 14) PF14295 - PAN domain (PAN_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACAATGGCCTAGGCCAACAAGAACCCG |
| Internal bar code: | CCCCGGCCCAGCAGTGGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 98.4326 |
| LEAP-Seq n confirming: | 314 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTTGTATGGGCTTGGGC |
| Suggested primer 2: | CATGCAATCATGTCCACCTC |