| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232219 |
| Chromosome: | chromosome 7 |
| Location: | 266945 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314000 | (1 of 1) PF16156 - Domain of unknown function (DUF4864) (DUF4864) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAAGAGACGCCTTAGGCTTATTGCAGG |
| Internal bar code: | TGCTTAGGAGGCAAACGGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 99.5724 |
| LEAP-Seq n confirming: | 1630 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTATGGCGGAGTTAGGC |
| Suggested primer 2: | TTTACAGTGAACGCAGTGGC |