| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232233 |
| Chromosome: | chromosome 10 |
| Location: | 4987982 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455250 | (1 of 6) PF02582 - Uncharacterised ACR, YagE family COG1723 (DUF155) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATCGCTTCTCCCAGCCCACCCTCCTCT |
| Internal bar code: | AGCGGTCCGATGGATGGTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 98.9287 |
| LEAP-Seq n confirming: | 6095 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTTGTTTTCGTTGGTAT |
| Suggested primer 2: | CAGATGAAGACAGGTGGGGT |