| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232274 |
| Chromosome: | chromosome 16 |
| Location: | 4178179 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668800 | MTA4 | Predicted protein; (1 of 2) PTHR24032:SF25 - SPORE COAT PROTEIN SP85 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGCGCAGCGCGCAGCGCACACACACCT |
| Internal bar code: | ATAACGGCTGCAGTATGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 461 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCATCTCTTTTTCGATGG |
| Suggested primer 2: | GGCTTGGAGCTGAAAGATTG |