Insertion junction: LMJ.RY0402.232276_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.232276
Chromosome:chromosome 1
Location:3833123
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g024800 (1 of 2) PF00059//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAACGTGAGCGCGTGCCCGACAGTATAC
Internal bar code:GTGTACTCCCGGAGTCGCTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:99.2131
LEAP-Seq n confirming:3152
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AATTCTGCGGGACGTAACAC
Suggested primer 2:ACATTCCGGTTTACCTGCTG