| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232280 |
| Chromosome: | chromosome 6 |
| Location: | 5317523 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283100 | FAP85 | Flagellar Associated Protein 85; (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGGTTGCACCCCGTCCATGATGGCGCC |
| Internal bar code: | GCAGGTTTTAATTCGGAATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 11.7647 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGCTAGATGCAGAGGTG |
| Suggested primer 2: | CTACTAGGCTTGACACGGGC |