| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232280 |
| Chromosome: | chromosome 17 |
| Location: | 3292531 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723100 | (1 of 10) PTHR31867:SF1 - EXPANSIN-A1-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGTCGGCCCGCTGGAATTGACCCTAAG |
| Internal bar code: | ACCTGGGGCGGCCCTGTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 98.8713 |
| LEAP-Seq n confirming: | 438 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAAGAGCCTGGAGGGGAA |
| Suggested primer 2: | ACCCTTCATGAATGCTCCAC |