| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232286 |
| Chromosome: | chromosome 10 |
| Location: | 90679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418250 | BLD10 | Basal body protein; (1 of 4) PTHR13140:SF289 - UNCONVENTIONAL MYOSIN-XIX | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGGTGGGAGACGGGCTGCCGGGGGTG |
| Internal bar code: | ACGTAATAACGCGACGCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 93.4615 |
| LEAP-Seq n confirming: | 243 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAGAGGTAAGGTGTCC |
| Suggested primer 2: | CACACCTGGCTGTCTAGCAA |