| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232290 |
| Chromosome: | chromosome 13 |
| Location: | 2191816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578350 | MPA11 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTACTTCACCCACTACAAGGAGATGGAT |
| Internal bar code: | TTCACAGGCGGGGGCCTTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.5992 |
| LEAP-Seq n confirming: | 497 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCGTGGCGAATACAGAT |
| Suggested primer 2: | ACTCCAGCGAGCTAACGTGT |