| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232299 |
| Chromosome: | chromosome 12 |
| Location: | 3833073 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515400 | (1 of 17) PF08016 - Polycystin cation channel (PKD_channel) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGCTGTGGGCACGACAGTGGTGCTGCC |
| Internal bar code: | GACAGCGAACTCGGGTAGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 98.2676 |
| LEAP-Seq n confirming: | 1645 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCATTTAACAGCTTCG |
| Suggested primer 2: | CTGATGCGCTACCTGCAATA |