Insertion junction: LMJ.RY0402.232305_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.232305
Chromosome:chromosome 15
Location:48611
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g634600 (1 of 1) PF12796//PF13637//PF13857 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) // Ankyrin repeats (many copies) (Ank_5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCATCTTTGCCGCGCCCCCCCGTCACCC
Internal bar code:ACGTGTGCGCGGTAGGACGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:99.2565
LEAP-Seq n confirming:4272
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTACTGCCACGTACACAC
Suggested primer 2:TACACGCCTCTCACACCTTG