Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.232352 |
Chromosome: | chromosome 6 |
Location: | 3567244 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g278118 | 5'UTR_intron|outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTAATGTACTATATAGGAATGCGTTGGG |
Internal bar code: | GAGTCTGGGGCTTGGGATCCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1181 |
LEAP-Seq percent confirming: | 99.3376 |
LEAP-Seq n confirming: | 4499 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGTAGGTAGCTTGGCGAG |
Suggested primer 2: | GGTAGCACAACAGGTGAGCA |