| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232352 |
| Chromosome: | chromosome 6 |
| Location: | 3567246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278118 | 5'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCCTAATGCAGTAAGAAATGGTACTTGT |
| Internal bar code: | AGGGACTGACGACCCGAGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 98.7646 |
| LEAP-Seq n confirming: | 1519 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTAGGTAGCTTGGCGAG |
| Suggested primer 2: | GGTAGCACAACAGGTGAGCA |