| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232355 |
| Chromosome: | chromosome 7 |
| Location: | 2748343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331300 | AGP3 | ADP-glucose pyrophosphorylase large subunit; (1 of 1) PF00132//PF00483 - Bacterial transferase hexapeptide (six repeats) (Hexapep) // Nucleotidyl transferase (NTP_transferase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACTCTCCCCCCTACATGCCCTCCCA |
| Internal bar code: | GCTTACAAGGGCAACCGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGGAGGAGGTAGAGGG |
| Suggested primer 2: | TCTTACGGAACATCAAGGGG |