Insertion junction: LMJ.RY0402.232389_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.232389
Chromosome:chromosome 16
Location:629866
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g692000 (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTGCTGCTGTTGTTGCCCTCGCTGCC
Internal bar code:GGGCGGTGGTAGGCAAACCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:36.2513
LEAP-Seq n confirming:352
LEAP-Seq n nonconfirming:619
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGGTTGGGTCTAGTCTGG
Suggested primer 2:CTATAGACCCTGTCTGCGCC