Insertion junction: LMJ.RY0402.232389_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.232389
Chromosome:chromosome 16
Location:630077
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g692000 (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGTACCGGGTGCGGCTGCTGTACAACCT
Internal bar code:AACGATACCGGAATACTGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:94
LEAP-Seq percent confirming:7.84133
LEAP-Seq n confirming:170
LEAP-Seq n nonconfirming:1998
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGACTTAATGACACGGTT
Suggested primer 2:CAACGGTAGTAGCAGTGGCA