| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232416 |
| Chromosome: | chromosome 14 |
| Location: | 2690503 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626576 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCCCCAAACCCTTCCCCCCCAAAAAC |
| Internal bar code: | AGAGGCAGTTTGCTTCACGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.1848 |
| LEAP-Seq n confirming: | 365 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGTGTTTTCGAGTGATG |
| Suggested primer 2: | AGAAATGCTTGGGGTGTTTG |