| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232420 |
| Chromosome: | chromosome_2 |
| Location: | 1351565 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g082950 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCCTGACAATAATACGGGAGTTATCTTT |
| Internal bar code: | AAACGCATTCGTCTTCGTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 99.2131 |
| LEAP-Seq n confirming: | 14625 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGACACATGCAGGCAG |
| Suggested primer 2: | CAGTAGCAGTAGCAGCACGC |