| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232432 |
| Chromosome: | chromosome 1 |
| Location: | 4278267 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028650 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTTGAAGGCGACATCACTTCCCGCCAC |
| Internal bar code: | CGCATCTGGCGATAATGGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 98.4153 |
| LEAP-Seq n confirming: | 8881 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 142 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCACAACAAAAATACGTG |
| Suggested primer 2: | AATCGGGAGGTTGTGTCTTG |