| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232448 |
| Chromosome: | chromosome 11 |
| Location: | 2773777 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCACTCACCCAGTTTTTCACCTTCAACT |
| Internal bar code: | GCGCCCGCGGCTGGTGGACATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 98.925 |
| LEAP-Seq n confirming: | 4141 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGGAGGTTGTGTGATG |
| Suggested primer 2: | CATAGCTGCACACGAAAGGA |