| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232463 |
| Chromosome: | chromosome 11 |
| Location: | 40470 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467527 | (1 of 1) PTHR24286:SF0 - CYTOCHROME P450 61 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAATAGAGGCCGCACGACCAACTATCAC |
| Internal bar code: | CTGTCTGAATTACTACGTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 99.3289 |
| LEAP-Seq n confirming: | 1332 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAAATTCTGCGTGGACC |
| Suggested primer 2: | TACGGGTCCTTCACCATCTC |