| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232519 |
| Chromosome: | chromosome 17 |
| Location: | 4001920 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728900 | FSA1,TAL3 | (1 of 1) PTHR10683//PTHR10683:SF2 - TRANSALDOLASE // SUBFAMILY NOT NAMED; Transaldolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTTTAAACAAACGACCAGAAGGTTTGC |
| Internal bar code: | GAACATGTCGCGTAGAGGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 96.1077 |
| LEAP-Seq n confirming: | 2321 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTCCTCGCCTATGTGG |
| Suggested primer 2: | TATGGGGCTGCAAGGATTAC |