| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232522 |
| Chromosome: | chromosome 1 |
| Location: | 3147775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019700 | PAP7 | Class-II RNA nucleotidyl transferase 7; (1 of 12) 2.7.7.19 - Polynucleotide adenylyltransferase / RNA adenylating enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATGAATGGGCACGCGCTGCCGAAGCCG |
| Internal bar code: | CCATGTTGGACAAGCGATAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 51037 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACAACCAGTTGGGAAGG |
| Suggested primer 2: | AGGATGAGGTTGCATTGCTC |