| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232560 |
| Chromosome: | chromosome 3 |
| Location: | 2136921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATGCCAGCTCATGCCGTACGACTCATT |
| Internal bar code: | CCGGCTACGTAGAACTGTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.0117 |
| LEAP-Seq n confirming: | 12322 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGATTTCTGGGTGCAACT |
| Suggested primer 2: | ATACGCGGTGTAGGGTTGAG |