| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232593 |
| Chromosome: | chromosome 11 |
| Location: | 3174816 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479383 | (1 of 1) IPR000104//IPR013122 - Antifreeze protein, type I // Polycystin cation channel, PKD1/PKD2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCCAATGCGGGAATGTCCAACACCATC |
| Internal bar code: | ACGTGAGTGAATAGTTCACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.6434 |
| LEAP-Seq n confirming: | 5588 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAAGGTGGTCCTCTGAC |
| Suggested primer 2: | AGTACGTACCGGTAGTGGCG |