Insertion junction: LMJ.RY0402.232603_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.232603
Chromosome:chromosome 8
Location:1632762
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g365500 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAAGACGCACACGCGGTCGGCCTCTAGG
Internal bar code:CGGTATTGGCACGCCAGGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:676
LEAP-Seq percent confirming:98.2811
LEAP-Seq n confirming:5203
LEAP-Seq n nonconfirming:91
LEAP-Seq n unique pos:123

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTCGGCATAATTGTGTG
Suggested primer 2:ACAACGGACAACTCCTCACC