| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232608 |
| Chromosome: | chromosome 7 |
| Location: | 5548142 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351450 | (1 of 1) PTHR14030:SF4 - MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 BETA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGGGGGTAGTTGCCGCGGAAGGGGTCA |
| Internal bar code: | CTTTTTCTGGTTCAGGTACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 99.5585 |
| LEAP-Seq n confirming: | 451 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGAGCCATAAGCAGGTC |
| Suggested primer 2: | AGGTTTGGGAGGACACACAG |