| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232625 |
| Chromosome: | chromosome 12 |
| Location: | 8597207 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTGCAGATAGGTGCGTTGCTCGCTTT |
| Internal bar code: | CTACGTAGTGTCGCGGTTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 93.5242 |
| LEAP-Seq n confirming: | 6109 |
| LEAP-Seq n nonconfirming: | 423 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGTCCGGTTAGGAGTGG |
| Suggested primer 2: | AGCAACATAAACACCCAGCC |