| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232653 |
| Chromosome: | chromosome 12 |
| Location: | 9195024 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541904 | (1 of 1) PF08755//PF13369 - Hemimethylated DNA-binding protein YccV like (YccV-like) // Transglutaminase-like superfamily (Transglut_core2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCCCCACAGGGAAGAGCACCGCCTCCT |
| Internal bar code: | CGATCGGCCACGCTAGGTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 69.6555 |
| LEAP-Seq n confirming: | 2548 |
| LEAP-Seq n nonconfirming: | 1110 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCAGTTAACCCCCACT |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |