| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232660 |
| Chromosome: | chromosome_3 |
| Location: | 7203372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g207900 | CYCA1 | A-type cyclin | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGCAGTGGGCACGGTCAGCTCGTACTTG |
| Internal bar code: | GGGTGTTCCAGGAGCACCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 98.2893 |
| LEAP-Seq n confirming: | 6550 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCGTACAGAGCCGTCAG |
| Suggested primer 2: | CACGATAAGAAACGCTGCAA |