| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232663 |
| Chromosome: | chromosome 6 |
| Location: | 7026525 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296850 | FAP81,DLEC1 | (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCACCACCGCATCAGCACAAATGCCTCC |
| Internal bar code: | CTACCGGGTTCCTGCTCCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 99.1549 |
| LEAP-Seq n confirming: | 1056 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCGAACTTCGGGTTAT |
| Suggested primer 2: | CAGCGAAAAGATCCCATCAT |