| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232672 |
| Chromosome: | chromosome 16 |
| Location: | 7540420 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688302 | (1 of 1) K08999 - hypothetical protein (K08999) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCTGCCGTCCCGAACCCAACCCCTGGC |
| Internal bar code: | TGGCGCTGCCATTGGATAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 98.949 |
| LEAP-Seq n confirming: | 9415 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACCAAGTCGGACATCA |
| Suggested primer 2: | ATGAAGGGCAGCAAGAAAGA |