Insertion junction: LMJ.RY0402.232676_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.232676
Chromosome:chromosome 11
Location:2623065
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g476376 FAP221,Pcdp1,RPC82 Flagellar Associated Protein 221; (1 of 1) PTHR23053//PTHR23053:SF21 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCCAGGCTGCCGTACGGGCTGTACGACG
Internal bar code:ACAGGGGCGCGGTTTCTTAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:474
LEAP-Seq percent confirming:99.4122
LEAP-Seq n confirming:111294
LEAP-Seq n nonconfirming:658
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCAGCCCAAACTGAACG
Suggested primer 2:TAGCGGTAAAGGAGGGGTTT