Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.232676 |
Chromosome: | chromosome_11 |
Location: | 2623073 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g476376 | FAP221 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCGTACAGCCGCGGCAGTACGACGTGTAC |
Internal bar code: | TGCGACTCATCGTGTAAAACCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 372 |
LEAP-Seq percent confirming: | 76.4706 |
LEAP-Seq n confirming: | 195 |
LEAP-Seq n nonconfirming: | 60 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATACCAGCCCAAACTGAACG |
Suggested primer 2: | TAGCGGTAAAGGAGGGGTTT |