| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232687 |
| Chromosome: | chromosome 12 |
| Location: | 3036259 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500715 | (1 of 5) 6.2.1.3 - Long-chain-fatty-acid--CoA ligase / Lignoceroyl-CoA synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGACATGTGCAGTGCACTTCTGCGTT |
| Internal bar code: | TTATGTATGTTTGTTCAATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 99.1498 |
| LEAP-Seq n confirming: | 3032 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGGAGCAGTCCCTCTAA |
| Suggested primer 2: | ACATGGTTCCGTCCTATTGC |