| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232701 |
| Chromosome: | chromosome 6 |
| Location: | 1322185 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258500 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCCTGTCCTAGGCAAGATGGCCTGTA |
| Internal bar code: | GTCGCTCGGTGGTTATGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1179 |
| LEAP-Seq percent confirming: | 98.745 |
| LEAP-Seq n confirming: | 16917 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTATCAACGCACAATGA |
| Suggested primer 2: | ATTATCGACTGTGCAAGGGC |