| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232743 |
| Chromosome: | chromosome 5 |
| Location: | 3009170 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGAGGCATCACCGATTGTTCGTACACT |
| Internal bar code: | AAAGGGAACGTAATAAAAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.2593 |
| LEAP-Seq n confirming: | 2412 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCACAACGTCACAAATG |
| Suggested primer 2: | TTCAACGCATGCTAGACCAG |