| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232755 |
| Chromosome: | chromosome 13 |
| Location: | 2234821 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578600 | (1 of 1) PTHR35464//PTHR35464:SF1 - FAMILY NOT NAMED // F18O14.9 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAAGCACGATGTGTAACAGCAAGTCTG |
| Internal bar code: | CGGGTTAGCCAAGCTATTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1271 |
| LEAP-Seq percent confirming: | 99.1459 |
| LEAP-Seq n confirming: | 4295 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGACAGGATTATTGGCTA |
| Suggested primer 2: | CGAGCGTATCCTTTTGCTTC |