| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232778 |
| Chromosome: | chromosome 2 |
| Location: | 7831264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142150 | (1 of 1) PF14770 - Transmembrane protein 18 (TMEM18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCATCACTTGGTCTTCTTCGCCTCAGG |
| Internal bar code: | GTGTAGTCAGGAAGGGGCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 97.6172 |
| LEAP-Seq n confirming: | 1229 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCAGGCAACCCAAATC |
| Suggested primer 2: | GCCACGTCTTACTCACCCAT |